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Terrific broth, modified, 500 g
Key Features and Details
Terrific Broth, Modified is a buffered enriched medium for the propagation of recombinant E. coli strains. The addition of extra Tryptone and Yeast Extract in the medium allows higher plasmid yield per volume. Glycerol is used as a carbohydrate source in this formulation. Unlike glucose, glycerol is not fermented to acetic acid.
SYNONYMS: TB modified
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PRODUCT DESCRIPTION
Terrific Broth, Modified is a buffered enriched medium for the propagation of recombinant E. coli strains. The medium was developed by Tartoff and Hobbs. Recombinant strains have an extended growth phase in the medium. The addition of extra Tryptone and Yeast Extract in the medium allows higher plasmid yield per volume. Glycerol is used as a carbohydrate source in this formulation. Unlike glucose, glycerol is not fermented to acetic acid. Terrific Broth, Modified has a lower concentraion of Dibasic Potassium Phosphate than Terrific Broth.
Application Notes
Terrific Broth, modified has been formulated for the cultivation of molecular genetic strains of Escherichia coli and increased plasmid yield.
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
Bacteria Cell Culture | Cultivation of molecular genetic strains of Escherichia coli and increased plasmid yield.
SPECIFICATIONS
SKU: 091010417
Base Catalog Number: 1010417
Alternate Names: TB modified
Format
: Powder
Formulation Details
: Powder | Following is composition of Terrific Broth; Modified in g/L: Tryptone - 12.0 g; Yeast extract - 24.0 g; Monopotassium phosphate - 2.2 g; Dipotassium phosphate - 9.4 g
Media Type
: Terrific broth
pH
: 7.2 ± 0.2 (3.5% solution)
Preparation Method
: Dissolve 47.6 g of the medium in 900 mL of deionized or distillled water. Add 8 mL of 50% glycerol. Mix well until evenly dispersed. Heat with frequent agitation and boil for one minute. Adjust final volume to 1000 mL. Sterilize in autoclave at 121 °C (15 lbs psi) for 15 minutes. Cool to room temperature. Store at 4 °C.
Sterility
: Sterilize in autoclave at 121 °C (15 lbs psi) for 15 minutes.
