In many cell culture applications using defined media for the in vitro cultivation of cells, the addition of supplemental nutrients and reagents is required. Additives such as antibiotics, buffers, and stains are frequently used to prevent bacterial contamination, control pH, and visibly monitor media conditions. Other supplements such as amino acids and vitamins are useful for enriched media beyond their normal concentrations. Attachment factors and transport factors are added to growth media to facilitate rapid cell propagation and enhance proper cell metabolism. Still other media supplements may be added to create conditions which will elicit specific cellular responses and effects, such as altered protein biosynthesis, accelerated antibody production and secretion, toxic response mechanisms, etc. As a result, the availability of a wide variety of media supplements is desirable when designing cell culture experiments.
Application Notes
Adding supplements of amino acids to media both stimulates growth and prolongs the viability of cells in culture. This concentrated supplement adds nutrients and reduces the biosynthetic burden on cell in vitro.
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
Mammalian Cell Culture | Stimulates growth and prolongs the viability of cells in culture