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Super Broth, 1 kg
Key Features and Details
Super Broth is a robust L-Broth variant, nutritionally rich medium developed for growth and maintenance of recombinant E. coli strains and generates higher plasmid densities. It has the formulation of 35 g Tryptone, 20 g Yeast Extract, 5 g NaCl
SYNONYMS: Luria Broth Base
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PRODUCT DESCRIPTION
SuperBroth is a robust L-Broth variant, nutritionally rich medium developed for growth and maintenance of recombinant E. coli strains and generates higher plasmid densities. It has the formulation of 35 g Tryptone, 20 g Yeast Extract, 5 g NaCl
Application Notes
L-Broth is a nutritionally rich medium originally developed for growth and maintenance of E. coli strains. L-Broth supports a luxurious growth of E. coli cells as tryptone and yeast extract supply essential growth factors that E. coli cells would otherwise have to synthesize. The medium also contains essential electrolytes for transport and osmotic balance, due to the NaCl component.
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
Cell culture |Proteomics |Purification and Detection |Recombinant Protein Expression and Analysis |Bacterial Culture |Plasmid Production |Protein Expression |Gene Expression |Cloning |Genetic Manipulation
SPECIFICATIONS
SKU: 113010032
Base Catalog Number: 3010032
Alternate Names: Luria Broth Base
Format
: Powder
Formulation Details
: Typically contain 35 g Tryptone; 20 g Yeast Extract; 5 g NaCl. Small quantities of biological buffers have been added for proper pH adjustment.
Grade
: Molecular Biology Certified
Media Type
: Super Broth
pH
: Acceptable pH range is 6.8 ± 0.3 at < 43 °C.
PPE
: Personal Protective Equipment Respiratory protection: Breathing apparatus only if aerosol or dust is formed. Hand protection: Pvc or other plastic material gloves Skin and body protection: Usual safety precautions while handling the product will provide adequate protection against this potential effect. Eye protection: Safety glasses with side-shields Hygiene measures: Handle in accordance with good industrial hygiene and safety practice.
Preparation Method
: Media Preparation: Reagents are tested and chosen for their ability to promote optimum growth of E. coli when combined in L-Broth Medium formulations. Chosen reagents are combined in the proper proportions according to the L-Broth formulation and thoroughly blended for a uniform distribution. A sample of the blended formulation is used to prepare liquid medium. The combinations are mixed to dissolve components and then autoclaved at 121 °C for 15 minutes.
Solubility
: Soluble in water (60 g/L).
Typical Working Concentration
: 60 g/L
