Traditional methods of plant cell wall disruption that rely on tissue pulverization in liquid nitrogen using a mortar and pestle are not needed when taking advantage of the Plant DNA Isolation Reagent. This plant DNA extraction kit makes preparing multiple samples at the same time more convenient. Furthermore, improvements in reagent composition reduce heat treatment time to only 15 minutes. In its entirety, the plant DNA extraction process can be completed within 30 minutes. Purified plant genomic DNA can be used directly for downstream applications such as PCR or restriction endonuclease digestion.