Using a straightforward RNA-isolation protocol, the CellAmp RT-PCR for Direct Prep Kit (Real Time) & Protein Analysis allows direct RNA template purification from animal cells for use in one- and two-step qRT-PCR. The kit enables RNA isolation from animal cells via a simple extraction method using animal cells cultured in 96-well (or other size well) plates. Direct RNA template purification from animal cells for use in real-time PCR takes approximately 10 minutes.