NucleoSpin Soil for the isolation of total DNA from soil microorganisms includes two alternative lysis buffers and an additional Enhancer SX, which can be combined with both lysis buffers. The resulting four different lysis systems make the kit suitable for all types of different samples, which is important because soil is very heterogeneous in pH and the amounts of organic or inorganic matter, such as humic substances or polysaccharides.