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Alcohol dehydrogenase
Key Features and Details
Alcohol Dehydrogenase
SYNONYMS: ADH; Alcohol : NAD+ oxidoreductase
CAS: #
9031-72-5
EC No: 232-870-4
MDL No: MFCD00081305
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PRODUCT DESCRIPTION
Alcohol Dehydrogenase
Application Notes
Alcohol dehydrogenase catalyzes the reaction: RCH2OH +NAD+ ? RCHO + NADH + H+ It facilitates the interconversion between alcohols and aldehydes or ketones with the reduction of nicotinamide adenine dinucleotide (NAD+ to NADH). In biotransformation, alcohol dehydrogenases are often used for the synthesis of enantiomerically pure stereoisomers of chiral alcohols.
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
Catalysis breakdown of ethanol | Synthesis of enantiomerically pure stereoisomers of chiral alcohols
SPECIFICATIONS
SKU: 02100161-CF
Base Catalog Number: 100161
Alternate Names: ADH; Alcohol : NAD+ oxidoreductase
Biochemical Physiological Actions
: Yeast alcohol dehydrogenase (yADH) serves as an excellent model system for enzyme-catalyzed H transfer because unlike many other enzymes, the chemical step, oxidation of a primary alcohol to an aldehyde by NAD+, is rate-limiting with aromatic substrates.
CAS
: #
9031-72-5
EC No
: 232-870-4
Extinction Coefficient
: E1%260= 12.6(lit.)
Format
: Powder
Grade
: 2× Crystallized
Isoelectric Point
: 5.4(lit.)
MDL No
: MFCD00081305
PPE
: Eyeshields, Gloves, respirator filter
Source
: Yeast
Specific Activity
: =300 u/mg protein
Specificity
: Yeast ADH which has a more narrow specificity than that of liver enzyme; accepts ethanol; is somewhat active on the straight chain primary alcohols; and acts to a very limited extent on certain secondary and branched chain alcohols. NADP does not serve as coenzyme.
Unit Definition
: One unit will convert 1.0 µmole of NAD/min at 25°C.
