Modified Oligos
Synbio Technologies provides oligo synthesis and modifications. Our specialized services include oligonucleotides modified with special bases, dU/dI, phosphorothioates, phosphorylation, spacers, amino linkers, disulfide linkers, biotin, etc. Synbio Technologies also provides multiple fluorescent groups and quenchers at 3’, 5’ or designated sites specified by the customer. This includes FAM, HEX, ROX, BHQ, Texas Red, and much more. The resulting oligos can be used in a variety of applications, including PCR, cloning, sequence verification, and gene detection.
Competitive Advantages
- Comprehensive Modifications and Labeling: Large-scale sequencing verifying the average mutation rate to be lower than 1/1000
- Strict Quality Control: Stringent ISO9001 & ISO13485 Certified Processing for oligo synthesis, meticulously monitored and advanced traceability
- Multiple Purification Options: Include desalt, PAGE Plus, PAGE, RP-HPLC, IE-HPLC and Dual HPLC
- Technical Support: Professional teams experienced in oligo synthesis and various modifications offer support throughout the production progress
Services Specifications
General Modifications
Single Labeling
Dual/Multiple Labeling
General Modifications
Synbio Technologies provides a series of general modifications with the high purity and competitive prices. We accomplish this by focusing on the requests of modification with special chemical classifications. Browse general modifications listed below:
| Classification | Modifications | Purification |
|---|---|---|
|
Modified Bases |
dU, dI, 5-Methyl dC |
HPLC/PAGE |
|
DBCO |
5’ DBCO, 3’ DBCO, dT-DBCO |
HPLC/PAGE |
|
AMCA |
5’ AMCA, 3’ AMCA, dT-AMCA |
HPLC/PAGE |
|
Phosphorothioates |
SPO3 |
HPLC/PAGE |
|
Amino Linkers |
5’Amino C6, dT-Amino C6, 3’Amino C7, 5’Amino C12 |
HPLC/PAGE |
|
Biotin |
5’Biotin, 3’Biotin, dT-Biotin |
HPLC/PAGE |
|
Digoxigenin |
5’Digoxigenin, 3’Digoxigenin, dT-Digoxigenin |
HPLC/PAGE |
|
Phosphorylation |
5’ PO4, 3’ PO4 |
HPLC/PAGE |
|
Hydrosulphonyl |
5’ HS-C6, 3’ HS-C3 |
HPLC/PAGE |
|
Locked Nuleic Acid |
LNA (A/G/C/U) |
HPLC/PAGE |
|
Fluoro Bases |
2’-F-2’-dA, 2’-F-2’-dC, 2’-F-2’-dG, 2’-F-2’-dU |
HPLC/PAGE |
|
Spacer |
dSpacer, Spacer C3, Spacer C9, Spacer C18 |
HPLC/PAGE |
Standard Deliverables
- Lyophilized dry DNA oligo powder
- 1 tube (Transparent or dark) or 96-well and 384-well plate
- Certificate of Analysis (COA) including sequencing information, OD, Tm, etc.
Single Labeling
Synbio Technologies provides multiple fluorescent groups at 3’, 5’ or designated sites to comply with our customers’ requests. This includes FAM, HEX, ROX, BHQ and Texas Red, etc.
| Dye/Quenchers | 3′- | 5′- | Designated Sites | Purification | |
|---|---|---|---|---|---|
|
FAM |
√ |
√ |
√ |
HPLC/PAGE |
|
|
TET |
√ |
√ |
√ |
HPLC/PAGE |
|
|
JOE |
√ |
√ |
√ |
HPLC/PAGE |
|
|
HEX |
√ |
√ |
√ |
HPLC/PAGE |
|
|
SIMA |
√ |
√ |
√ |
HPLC/PAGE |
|
|
TAMRA |
√ |
√ |
√ |
HPLC/PAGE |
|
|
ROX |
√ |
√ |
√ |
HPLC/PAGE |
|
|
Cy5 |
√ |
√ |
√ |
HPLC/PAGE |
|
|
Cy3 |
√ |
√ |
√ |
HPLC/PAGE |
|
|
Quasar670 |
√ |
√ |
|
HPLC/PAGE |
|
|
Quasar570 |
√ |
√ |
|
HPLC/PAGE |
|
|
DyLight547 |
|
√ |
|
HPLC/PAGE |
|
|
DyLight647 |
|
√ |
|
HPLC/PAGE |
|
|
Texas Red |
|
√ |
|
HPLC/PAGE |
|
|
BHQ-1 |
√ |
|
|
HPLC/PAGE |
|
|
BHQ-2 |
√ |
|
|
HPLC/PAGE |
|
|
BHQ-3 |
√ |
|
|
HPLC/PAGE |
|
|
Eclipse |
√ |
|
|
HPLC/PAGE |
|
|
DABCYL |
√ |
|
|
HPLC/PAGE |
Standard Deliverables
- Lyophilized dry DNA oligo powder
- 1 tube (Transparent or dark) or 96-well and 384-well plate
- Certificate of Analysis (COA) including sequencing information, OD, Tm, etc.
Dual/Multiple Labeling
Despite of single fluorescent group, Synbio Technologies also offers dual/multiple labeling services including TAMRA, BHQ, DABYCL, ELCISPE, etc. The most common Dyes and Quenchers are listed below:
| Dyes | Quenchers | Purification |
|---|---|---|
|
5’6-FAM |
3’TAMRA, 3’BHQ-1, 3’Eclipse |
HPLC/PAGE |
|
5’TET |
3’TAMRA, 3’BHQ-2, 3’DABCYL |
HPLC/PAGE |
|
5’HEX |
3’TAMRA, 3’BHQ-1, 3’BHQ-2, 3’DABCYL |
HPLC/PAGE |
|
5’TAMRA |
3’Eclipse, 3’BHQ-1, 3’BHQ-2, 3’DABCYL |
HPLC/PAGE |
|
5’ROX |
3’Eclipse, 3’BHQ-1, 3’BHQ-2, 3’DABCYL |
HPLC/PAGE |
|
5’CY5 |
3’BHQ-1, 3’BHQ-2, 3’BHQ-3, 3’DABCYL |
HPLC/PAGE |
|
5’CY3 |
3’BHQ-2 |
HPLC/PAGE |
|
5’JOE |
3’Eclipse, 3’BHQ-1, 3’BHQ-2. 3’DABCYL, 3’TAMRA |
HPLC/PAGE |
|
5’JOE |
3’BHQ-2 |
HPLC/PAGE |
Standard Deliverables
- Lyophilized dry DNA oligo powder
- 1 tube (Transparent or dark) or 96-well and 384-well plate
- Certificate of Analysis (COA) including sequencing information, OD, Tm, etc.
