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Hank’s Balanced Salts (Hbs) (modified) (1X) w/o Ca, Mg, phenol red, 500 mL
Hank’s Balanced Salts (HBS) (Modified) (1X Solution) without calcium, magnesium, phenol red
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1XHanks’ Balanced Salts (modified), w/o Mg and Ca, 500 mL
1× Hanks’ Balanced Salts (Modified), without Magnesium and Calcium
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1X Hanks’ Balanced Salt Solution (modified) w/o phenol red, 500 mL
Hanks’ balanced salt solution without phenol red
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1X Hanks’ Balanced Salt Solution, modified, 500 mL
1× Hanks’ Balanced Salt Solution, Modified
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Earle’s Balanced Salts (modified) (EBS) (1X) w/o phenol red, 500 mL
Earle’s Balanced Salts (Modified) (EBS) (1X Solution) without phenol red
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Phosphate Buffered Saline (PBS), Dulbecco’s formula, powder, w/o Ca, Mg
Phosphate Buffered Salts, Dulbecco’s Formula (DPBS) is a balanced salt solution (BSS) used for the handling and culturing of mammalian cells. DPBS is used to irrigate, wash, and dilute mammalian cells. Phosphate buffering maintains the pH in the physiological range. Calcium and magnesium facilitate cell binding and clumping. DPBS without these ions can be used to wash and rinse suspended cells.
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Hanks’ Balanced Salts, powder, w/o sodium bicarbonate
Hanks’ Balanced Salts solution is used to maintain the pH and osmotic balance in the medium and to provide the cells with water and essential inorganic ions.
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Earle’s Balanced Salts w/o sodium bicarbonate, 10 L
Earle’s Balanced Salts without Sodium Bicarbonate.
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Water for cell culture, 500 mL
For use in the preparation of cell culture media, and cell suspension and washing solutions.
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Phenol red, 0.5% solution, 100 mL
Phenol red is an acid-base indicator. It is made by condensing two moles of phenol with one mole of o-sulfobenzoic acid anhydride. Phenol red in tissue culture media can act as a weak estrogen, especially with human breast cancer cells. Phenol red can be used to measure hydrogen peroxide in cultured macrophages in multiwell plates.
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1X Trypsin-EDTA solution, 100 mL
0.25% Trypsin (1:250) and 1mM EDTA in modified HBSS w/o Ca++ and Mg++, 100 mL
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HEPES buffer, 1 M, 100 mL
Ready to use solution
Cell culture grade -
Sodium Ppruvate, 100 mM (11 mg/mL), 100 mL
Sodium pyruvate is used by cells as an easily accessible carbohydrate source. The 100 mM solution should be diluted 1:100 for most cell culture.
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Non-essential amino acids for Minimum Essential Medium Eagle, 100X, 100 mL
100X Non-essential Amino Acids for Minimum Essential Medium Eagle Contains 19 amino acids. The essential amino acids and the non-essential amino acids; L-ala; L-asn; L-asp; L-glu; L-gly; L-pro and L-ser.
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HT, hypoxanthine/thymidine solution (50X solution), 100 mL
This media is selective for growth of cells with a functioning HGPRT mechanism. The main application is for the selective growth of hybridoma cells for monoclonal antibody production. (50X, 13.61 mg/L hypoxanthine, 3.876 mg/L thymidine)
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Hat supplement (50X solution), 100 mL
Selective for hybridoma cell growth and growth of cells with a functioning HGPRT mechanism. The main application is for the selective growth of hybridoma cells for monoclonal antibody production. (50x formulation: 13.61 mg/L hypoxanthine, 0.1906 mg/L aminopterin 2H2O,3.876 mg/L thymidine.)
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L-glutamine (frozen), 200 mM, 100 mL
L-glutamine is an essential amino acid that is a crucial component of culture media that serves as a major energy source for cells in culture.
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Trace element solution 2 (1000X), 100 mL
MP Biomedicals offers a 1000X sterile filtered solution for use with serum-free media. It has the same composition as Clevelands Trace Element I.
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RPMI 1640 with 2 g/L sodium bicarbonate, w/o L-glutamine & glucose, 500 mL
General purpose medium based on the RPMI 1630 line of media
A Dutch Modification of RPMI 1640 is also available through MP -
RPMI 1640 (1X) w/o L-glutamine, phenol red, 500 mL
RPMI 1640 medium is a general purpose medium based on the RPMI 1630 line of media which utilized a bicarbonate buffering system and alterations in the amounts of amino acids and vitamins. Without L-glutamine, phenol red; Endotoxin Tested; Sterile Filtered
