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LymphoSepMate Lymphocyte Isolation Kit, 50 mL, 25 Tubes, Sterile
Ready-to-use with 10 mL lymphocyte separation medium pre-loaded in a 50 mL centrifuge tube with a porous membrane for optimal separation of lymphocytes and mononuclear cells from blood and tissues.
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LymphoSepMate Lymphocyte Isolation Kit, 15 mL, 25 Tubes, Sterile
Ready-to-use with 3 mL lymphocyte separation medium pre-loaded in a 15 mL centrifuge tube with a porous membrane for optimal separation of lymphocytes and mononuclear cells from blood and tissues.
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LymphoSepMate Lymphocyte Isolation Tube, 50 mL, 25 Tubes, Sterile
An innovative separation tube with a porous membrane for optimal separation of lymphocytes and mononuclear cells from blood and tissues.
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LymphoSepMate Lymphocyte Isolation Tube, 15 mL, 25 Tubes, Sterile
An innovative separation tube with a porous membrane for optimal separation of lymphocytes and mononuclear cells from blood and tissues.
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LymphoSep, lymphocyte separation medium
Lymphocyte Separation Medium is a sterile, iso-osmotic, low viscosity solution for density centrifugation yielding a clear separation of lymphocytes from heparinized or defibrinated human blood.
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Neutral red solution, 100 mL
1:300 Solution. Used as a red nuclear counterstain in various cytochemical procedures.
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Trypan blue, 0.4% solution in PBS, 100 mL
Trypan Blue is a blue acid dye with a strong affinity for cellulose. Trypan blue solution may be used in trypan blue based cytotoxitiy and proliferation assays. Trypan blue is also recommended for use in dye exclusion procedures for viable cell counting. Non-viable cells will up-take trypan blue at a faster rate than viable cells.
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Phenol red, 0.5% solution, 100 mL
Phenol red is an acid-base indicator. It is made by condensing two moles of phenol with one mole of o-sulfobenzoic acid anhydride. Phenol red in tissue culture media can act as a weak estrogen, especially with human breast cancer cells. Phenol red can be used to measure hydrogen peroxide in cultured macrophages in multiwell plates.
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1X Trypsin-EDTA solution, 100 mL
0.25% Trypsin (1:250) and 1mM EDTA in modified HBSS w/o Ca++ and Mg++, 100 mL
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10X Trypsin HBSS w/o calcium and magnesium, 100 mL
Trypsin is a porcine pancreas-derived enzyme that is commonly used for the dissociation and disaggregation of anchorage-dependent mammalian cells and tissues.
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1X Trypsin-EDTA solution (0.05% (w/v) Trypsin (1:250) and 0.02% (w/v) EDTA), 100 mL
Used for cell dissociation, routine cell culture passaging, and primary tissue dissociation due to its digestive strength.0
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HEPES buffer, 1 M, 100 mL
Ready to use solution
Cell culture grade -
Sodium Ppruvate, 100 mM (11 mg/mL), 100 mL
Sodium pyruvate is used by cells as an easily accessible carbohydrate source. The 100 mM solution should be diluted 1:100 for most cell culture.
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Non-essential amino acids for Minimum Essential Medium Eagle, 100X, 100 mL
100X Non-essential Amino Acids for Minimum Essential Medium Eagle Contains 19 amino acids. The essential amino acids and the non-essential amino acids; L-ala; L-asn; L-asp; L-glu; L-gly; L-pro and L-ser.
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HT, hypoxanthine/thymidine solution (50X solution), 100 mL
This media is selective for growth of cells with a functioning HGPRT mechanism. The main application is for the selective growth of hybridoma cells for monoclonal antibody production. (50X, 13.61 mg/L hypoxanthine, 3.876 mg/L thymidine)
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Hat supplement (50X solution), 100 mL
Selective for hybridoma cell growth and growth of cells with a functioning HGPRT mechanism. The main application is for the selective growth of hybridoma cells for monoclonal antibody production. (50x formulation: 13.61 mg/L hypoxanthine, 0.1906 mg/L aminopterin 2H2O,3.876 mg/L thymidine.)
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L-glutamine (frozen), 200 mM, 100 mL
L-glutamine is an essential amino acid that is a crucial component of culture media that serves as a major energy source for cells in culture.
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Trace element solution 2 (1000X), 100 mL
MP Biomedicals offers a 1000X sterile filtered solution for use with serum-free media. It has the same composition as Clevelands Trace Element I.
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Trace element solution 1 (1000X), 100 mL
MP Biomedicals offers a 1000X sterile filtered solution for use with serum-free media. It includes selenium, copper, zinc, and iron, as well as ferric citrate for greater stability.
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Gentamicin sulfate, 50 mg/mL
Gentamicin is an aminoglycoside antibiotic complex that inhibits the growth of a wide variety of Gram-positive and Gram-negative microorganisms, including strains resistant to tetracycline, chloramphenicol, kanamycin, and colistin, particularly strains of Pseudomonas, Proteus, Staphylococcus, and Streptococcus. It is effective against mycoplasma as well.
