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EasyPure Plasmid MiniPrep Kit by TransGen Biotech is a reagent kit designed for fast, highyield isolation of highquality plasmid DNA from E.coli bacterial cultures, using alkaline lysis and silica membrane technology, featuring colored indicators in buffers for visual confirmation of lysis neutralization, yielding up to 40ug DNA for applications like sequencing and cloning.
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EasyPure Plasmid MiniPrep Kit uses a modified alkaline lysis method followed by adsorption of DNA onto silica membrane to isolate high-quality plasmid DNA from ≤20ml cultures of E.coli in LB medium. DNA yield up to 40µg.
The solution contains indicators that can indicate whether the lysis and neutralization are complete through the change of color, so as to ensure the quality of plasmid extraction and visualize the operation.
The purified plasmid DNA is suitable for a variety of molecular biology applications, including restriction enzyme digestion, ligation, transformation and DNA sequencing.
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