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3,3' Diaminobenzidine tetrahydrochloride, 100 mg
Key Features and Details
SYNONYMS: DAB
CAS: # 7411-49-6
MOLECULAR FORMULA: C
12
H
14
N
4
*4HCl
MOLECULAR WEIGHT: 360.10
BEILSTEIN REGISTRY No.: 1212988
EC No: 231-018-9
MDL No: MFCD00012969
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PRODUCT DESCRIPTION
SPECIFICATIONS
SKU: 0215082680
Base Catalog Number: 15082680
Alternate Names: DAB
Beilstein Registration No
: 1212988
CAS
: # 7411-49-6
EC No
: 231-018-9
Hazard Statements
: H341-H350
MDL No
: MFCD00012969
Melting Point
: > 300 °C (Lit.)
Molecular Formula
: C
12
H
14
N
4
*4HCl
Molecular Weight
: 360.10
PPE
: Eyeshields, Gloves, respirator filter
Preparation Method
: Typical Preparation of solutions for Peroxidase Detection : Dissolve 12 mg of DAB in 1.0 mL 50 mM Tris buffer, pH 7.3 (or inject 8.3 mL of buffer into serum vials if using #150826). Working size aliquots may be stored for 6 months at -20 °C. The stock solution is diluted 1:20 with 50 mM Tris buffer pH 7.3 just before developing the immunoblot. To 10 mL add 10 uL 30% hydrogen peroxide. Add 10 mL of this substrate solution per 15 x 15 cm2 membrane. Develop the blot at room temperature with agitation until the bands are suitably dark. This typically requires 1-5 min. Stop the reaction by rinsing away the substrate with PBS.
RTECS No
: DV8753000
Safety Symbol
: GHS08
Solubility
: Easily soluble in water. The aqueous solution is unstable and can be used only for a few days even if it is kept in a refrigerator. 1% aqueous solution is dark and turns reddish when exposed to light.
