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AlbumiNZ Bovine Albumin Low Endotoxin, =97%
Key Features and Details
Stem Cell Grade
From New Zealand-sourced bovine plasma
CAS: #
9048-46-8
MOLECULAR WEIGHT: 66KDa
EC No: 232-936-2
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PRODUCT DESCRIPTION
Bovine albumin is a single polypeptide chain consisting of approximately 583 to 595 amino acid residues and no carbohydrates. It contains 17 intrachain disulfide bridges and 1 sulfhydryl group
Application Notes
Albumin binds water, Ca2+, Na+, and K+. Due to a hydrophobic cleft, albumin binds fatty acids, bilirubin, hormones and drugs. The main biological function of albumin is to regulate the colloidal osmotic pressure of blood and to a lesser degree to provide cellular nutrition. Bovine albumin contains 16% nitrogen and is often used as a standard in protein calibration studies. Albumin is used to solubilize lipids, stabilize protein solutions, and is also used as a blocking agent in Western blots or ELISA applications.
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
Stem cell research | Cell Culture applications | Immunoassay
SPECIFICATIONS
SKU: 02199896-CF
Base Catalog Number: 199896
Background Information
: Albumins are a group of acidic proteins which occur in the body fluids and tissues of mammals and in some plant seeds. Serum and plasma albumin is carbohydrate-free and comprises 55-62% of the protein present. However, only about 40% of the total albumin in the body is in the circulating plasma at one time with the remainder being in extracellular spaces with which there is, in general, equilibration about every 24 hours.
Biochemical Physiological Actions
: Albumin loses its helical structure rapidly in 2-4 M urea and 4 M guanidinium chloride. The unfolding is reversible, even, to some extent, after concomitant reduction of all disulfide bonds . Reduced albumin can reform into structures capable of binding antibodies and other ligands . Formation of SS bonds is rapid but the return of native structure is slow and some wrong disulfide pairings may persist . After oxidation of the cystine bridges, however, native structure is irreversibly lost.
CAS
: #
9048-46-8
Concentration
: Total Protein: =95%
EC No
: 232-936-2
Format
: Lyophilized powder
Grade
: Cell Culture , ImmunoAssay (EIA)
Host
: Bovine
Molecular Weight
: 66KDa
pH
: 6.5 - 7.5 (4% aq soln)
Purity
: =97% Total Protein
Solubility
: Albumins are readily soluble in water and can only be precipitated by high concentrations of neutral salts such as ammonium sulfate. The solution stability of albumin is very good (especially if the solutions are stored aliquoted and frozen). Albumin is readily coagulated by heat. When heated to 50 °C or above, albumin quite rapidly forms hydrophobic aggregates which do not revert to monomers upon cooling. Dissolves completely in 20 min at 20-25 °C (20%).
