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Ampicillin, trihydrate
Key Features and Details
Ampicillin Trihydrate
SYNONYMS: Aminobenzylpenicillin trihydrate; alpha-Aminobenzylpenicillin trihydrate ;D[-]-a-Aminobenzylpenicillin; 6-[D(-)-a-Aminophenylacetamido]penicillanic acid
CAS: #
7177-48-2
MOLECULAR FORMULA: C16H19N3O4S·3H2O
MOLECULAR WEIGHT: 403.45 g/mol
BEILSTEIN REGISTRY No.: 5399534
EC No: 200-709-7
MDL No: MFCD00072036
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PRODUCT DESCRIPTION
Ampicillin Trihydrate
Application Notes
Ampicillin Trihydrate is commonly used to select for ampicillin resistance in mutated and transformed cells. It is also described for the use of inhancing luminol chemiluminescence.
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
Antibiotic
SPECIFICATIONS
SKU: 02190147-CF
Base Catalog Number: 190147
Alternate Names: Aminobenzylpenicillin trihydrate; alpha-Aminobenzylpenicillin trihydrate ;D[-]-a-Aminobenzylpenicillin; 6-[D(-)-a-Aminophenylacetamido]penicillanic acid
Beilstein Registration No
: 5399534
Biochemical Physiological Actions
: A ß-lactam antibiotic with an amino group side chain attached to the penicillin structure. Penicillin derivative that inhibits bacterial cell-wall synthesis (peptidoglycan cross-linking) by inactivating transpeptidases on the inner surface of the bacterial cell membrane. Bactericidal only to growing Escherichia coli. Mode of resistance: Cleavage of ß-lactam ring of ampicillin by ß-lactamase. Antimicrobial spectrum: Gram-negative and Gram-positive bacteria.
CAS
: #
7177-48-2
EC No
: 200-709-7
Format
: Powder
Hazard Statements
: H315-H317-H319-H334-H335
MDL No
: MFCD00072036
Melting Point
: 388 to 392° F (decomposes) (NTP, 1992)
Molecular Formula
: C16H19N3O4S·3H2O
Molecular Weight
: 403.45 g/mol
pKa
: At 25 °C ,pKa = 2.5 (-COOH),pKa = 7.3 (-NH2) (Lit.)
PPE
: Dust mask, Eyeshields, Faceshields, Gloves
RTECS No
: XH8425000
Safety Symbol
: GHS07, GHS08
Solubility
: 1 to 10 mg/mL at 70° F (NTP, 1992)
Sterilization of Solutions
: Autoclaving solutions of ampicillin will destroy activity. Solutions can be sterilized by filtration (0.22 um filter) and stored frozen (-20 °C or below). Frozen solutions can be stored for up to 3 months.
Typical Working Concentration
: 100 mg/liter for both gram positive and gram negative bacteria. It is typically stable in media at 37 °C for approximately 3 days.
