Bovine Brain Extract (BBE) is made from fresh, unfrozen pituitary glands from young, healthy cattle less than 30 months old. They are born, raised and slaughtered in New Zealand from a USDA approved source. New Zealand cattle are internationally recognized for being free from bovine spongiform encephalopathy (BSE, mad-cow disease) and have no history of blue tongue disease.
This Bovine Brain Extract (BBE) is formulated to be used at 0.1-0.2 % in the final serum-free medium. Studies have shown this level of BBE at the standard 7.5-15 micrograms/ mL will fully replace endothelial cell growth supplement (ECGS). BBE allows culture of these cells without feeder layers.
Application Notes
Bovine Brain Extract (BBE) is formulated to be used at 0.1-0.2 % in the final serum-free medium (i.e. use 0.5-1 mL BBE for 500 mL medium). Studies have shown this level of BBE at the standard 7.5-15 micrograms/ mL will fully replace endothelial cell growth supplement (ECGS). BBE allows culture of these cells without feeder layers.
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.