Bovine pituitary extract

Bovine Pituitary Extract (BPE) is made from fresh, flash-frozen pituitary glands from young, healthy cattle between the ages of 18-22 months. The cattle are born, raised and slaughtered in New Zealand from a USDA approved source. New Zealand cattle are internationally recognized as free from bovine spongiform encephalopathy (BSE, mad-cow disease) and have no history of blue tongue disease. BPE continues to be the most successful of the tissue-sourced extracts to date and allows researchers to study normal primary cell types including stem cells in proliferating cultures. Formulated at 14 mg/ mL crude protein, this aqueous BPE has a full spectrum of mitogens and differentiation factors. The Bovine Pituitary Extract is not lyophilized, as this process has been shown to reduce biological activity. For cell culture, the optimal medium supplementation rate is 35 µg/mL BPE based on growth tests on human mammary epithelial cells, epidermal keratinocytes, prostatic and bronchial epithelial cells. This product has been sterile-filtered prior to shipping. Application Notes 35-70 µg/ mL or 1.25-2.5 mL per 500 mL of medium for monolayer growth of epithelial cells. Optimal concentration may vary depending on cell type. Usage Statement Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.