Collagenase type II, >125 U/mg solid

Key Features and Details

From Clostridium histolyticum
Protease which cleaves collagen
For use in the preparation of isolated cell suspensions

SYNONYMS: Clostridiopeptidase A
CAS: # 2593923
EC No: 232-582-9
MDL No: MFCD00130830

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PRODUCT DESCRIPTION

Collagenase Type Ii Application Notes Collagenase used for collagen structural and biosynthetic studies. For tissue dissociation most researchers employ either crude collagenase preparations or chromatographically purified collagenase combined with other enzymes such as elastase, trypsin, and/or papain. Collagenase from Clostridium histolyticum has been used in a study to investigate the survivability of collagen micronetworks in the presence of collagenase. Collagenase from Clostridium histolyticum has also been used in a study to investigate the degradation of collagen by the cariogenic bacteria, Streptococcus mutans. Usage Statement Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. Key Applications Release of cells from animal tissues
SPECIFICATIONS
SKU: 02100502-CF
Base Catalog Number: 100502
Alternate Names: Clostridiopeptidase A
CAS : # 2593923
EC No : 232-582-9
Format : Lyophilized powder
Hazard Statements : H315-H319-H334-H335
MDL No : MFCD00130830
PPE : Dust mask, Eyeshields, Faceshields, Gloves
Safety Symbol : GHS07, GHS08
Source : Clostridium histolyticum
Specific Activity : >125 u/mg solid
Specificity : Clostridial collagenase I or clostridiopeptidase A degrades the helical regions in native collagen preferentially at the Y-Gly bond in the sequence -Pro-Y-Gly-Pro- where Y is most frequently a neutral amino acid. This bond in synthetic peptide substrates may also be split.
Unit Definition : One unit liberates one µmole of L-leucine equivalents from collagen in 5 hours at 37 °C, pH 7.5

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