CSM-HIS, powder

Single dropout formulation ( without L-Histidine) of Complete Supplement Mixture (CSM) of amino acids for S. cerevisiae growth media. Time tested formulation is available in complete and dropout formulations. Application Notes The selection of plasmids in yeast is based on the use of auxotrophic mutant strains that cannot grow without a specific media component (an amino acid, purine or pyrimidine). Transformation with a plasmid containing the mutated gene enables the transformant to grow on a medium lacking the required component. This CSM-Dropout medium create a richer medium for better yield and growth rate, and increase the probability of successful transformations when screening libraries or performing gene knock-outs. Usage Statement Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. Key Applications Cell culture |Proteomics |Purification and Detection |Protein Analysis |Yeast Culture |Plasmid Production |Protein Expression |Gene Expression |Cloning |Gene Function |Gene Regulation |Automated Bacterial Growth Media |S. cerevisiae Culture

Formulation Details : Contents per Liter: Adenine 10* mg |L-Arginine HCl 50 mg |L-Aspartic Acid 80 mg |L-Histidine HCl 0 mg |L-Isoleucine 50 mg |L-Leucine 100 mg |L-Lysine HCl 50mg |L-Methionine 20** mg |L-Phenylalanine 50 mg |L-Threonine 100** mg |L-Tryptophan 50 mg |Uracil 20 mg| L-Tyrosine 50 mg |L-Valine 140 mg |Total 770.000 |*Minimum quantity for healthy growth and yet optimized to promote red color in certain adenine auxotrophs. CSM formulations are available that contain 20 or 40 mg/liter adenine. **80mg/liter of DL-Homoserine is substituted for L-Threonine in mixtures where L-Methionine is dropped out.