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Deoxyribonuclease I, =2000 kunitz U/mg
Key Features and Details
Deoxyribonuclease I
SYNONYMS: DNase I; Deoxyribonucleate 5'-oligonucleotido-hydrolase
CAS: #
9003-98-9
EC No: 232-667-0
MDL No: MFCD00130918
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PRODUCT DESCRIPTION
Deoxyribonuclease I
Application Notes
DNase I is used as a genetic marker for forensic identification. Used for the removal of DNA from protein samples. DNAse I is used to nick DNA, as a first step to incorporate labeled bases into DNA. The enzyme from Sigma has been used in the nuclease stock along with RNAse during cell lysate preparation of Madin-Darby canine kidney (MDCK) II cell lines. It has also been used during RNA extraction from Sindbis virus. Deoxyribonuclease I from bovine pancreas has been used for the identification, localization and expression of two novel human genes similar to deoxyribonuclease I. Deoxyribonuclease I from bovine pancreas has also been used in a study to investigate a new approach to obtaining high-activity RNase, DNase, cholesterolesterase, and trypsin from cattle pancreas.
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
Marker | Cell lysis
SPECIFICATIONS
SKU: 02100575-CF
Base Catalog Number: 100575
Alternate Names: DNase I; Deoxyribonucleate 5'-oligonucleotido-hydrolase
Biochemical Physiological Actions
: DNase I is an endonuclease that acts on phosphodiester bonds adjacent to pyrimidines to produce polynucleotides with terminal 5'-phosphates. In the presence of Mg2+, DNAse I cleaves each strand of DNA independently and the cleavage sites are random. Both DNA strands are cleaved at approximately the same site in the presence of Mn2+. The pH optimum is found to be between 7 and 8. Divalent cations such as Mn2+, Ca2+, Co2+, and Zn2+ are activators of the enzyme. A concentration of 5 mM Ca2+ stabilizes the enzyme against proteolytic digestion. DNAse I from bovine pancreas consists of four chromatographically distinguishable components, A, B, C, and D, with their molar ratios being 4:1:1 respectively. Only minor amounts of D are found. 2-Mercaptoethanol, chelators, sodium dodecyl sulfate (SDS) and actin are known to inhibit the enzyme activity.
CAS
: #
9003-98-9
EC No
: 232-667-0
Extinction Coefficient
: E1%280= 11.1.
Formulation Details
: A lyophilized powder containing a small amount of glycine-stabilizer
MDL No
: MFCD00130918
RTECS No
: RF0750000
Source
: Bovine Pancreas
Specific Activity
: =2000 kunitz u/mg dw
Unit Definition
: One unit causes an increase in absorbance at 260nm of 0.001/minute per ml at 25°C, pH 5.0, when acting on highly polymerized DNA in the presence of ionized magnesium and calcium.
