DL-Dithiothreitol

Key Features and Details

Effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE
Useful for stabilizing sulfhydryl containing enzymes
Less pungent and is less toxic than 2-mercaptoethanol

SYNONYMS: 1,4-Dithiothreitol; DTT; 1,4-Dithio-DL-threitol; Cleland Reagent; (±)-threo-1,4-Dimercapto-2,3-butanediol
CAS: # 27565-41-9
MOLECULAR FORMULA: C4H10O2S2
BEILSTEIN REGISTRY No.: 1719757
EC No: 222-468-7
MDL No: MFCD00004877
SKU 04856126-CF Categories , , Tag

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PRODUCT DESCRIPTION

Dl-Dithiothreitol Application Notes Dithiothreitol is useful for stabilizing sulfhydryl containing enzymes. Effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE. DTT can also be used for reducing the disulfide bridge of the cross-linker N,N'-bis(acryloyl)cystamine to break apart the matrix of a polyacrylamide gel. DTT is less pungent and is less toxic than 2-mercaptoethanol. Usage Statement Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. Key Applications Sulfhydryl reducing agent | Blotting | Protein Purification
SPECIFICATIONS
SKU: 04856126-CF
Base Catalog Number: 856126
Alternate Names: 1,4-Dithiothreitol; DTT; 1,4-Dithio-DL-threitol; Cleland Reagent; (±)-threo-1,4-Dimercapto-2,3-butanediol
Beilstein Registration No : 1719757
CAS : # 27565-41-9
EC No : 222-468-7
Format : Powder
Grade : Electrophoresis
Half Life : 1.4( pH 8.5 at 2 °C)
Hazard Statements : H302-H315-H319-H335
MDL No : MFCD00004877
Molecular Formula : C4H10O2S2
PPE : Dust mask , Eyeshields, Faceshields, Gloves
Purity : =99.5%
RTECS No : EK1610000
Safety Symbol : GHS07
Typical Working Concentration : Typically, a seven fold lower concentration of DTT (100 mM) is needed than is used for 2-mercaptoethanol (5% v/v, 700 mM).

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