Dulbecco's Phosphate Buffered Saline (10X), w/o calcium and magnesium, 500 mL

Role of a balanced salt solution in cell culture is multifaceted and can be divided into four principal functions: 1. Serves as an irrigating, transporting and diluting fluid while maintaining intra- and extracellular osmotic balance; 2. Provides cells with water and certain bulk inorganic ions essential for normal cell metabolism; 3. Combined with a carbohydrate, such as glucose, provides the principal energy source for cell metabolism; 4. Provides a buffering system to maintain the medium within the physiological pH range (7.2 - 7.6) Application Notes Dulbecco's Phosphate Buffered Saline 10X is a stock solution used to prepare 1X D-PBS in cell culture grade water. D-PBS is used to wash cells during preparation and serial transfer Phosphate buffering maintains the pH in the physiological range. Calcium and magnesium facilitate cell binding and clumping. DPBS without these ions can be used to wash and rinse suspended cells. Used to wash cells during preparation and serial transfer. Usage Statement Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. Key Applications Mammalian Cell Culture

Formulation Details : Liquid | (as 10X) Inorganic Salts: Potassium Chloride [KCl]: 2000.00 mg/L; Potassium Phosphate Monobasic [KH₂PO₄]: 2000.00 mg/L; Sodium Chloride [NaCl]: 80000.00 mg/L; Sodium Phosphate Dibasic [Na₂HPO₄]: 11500.00 mg/L