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EMM no nitrogen (no NH4CL, glutamate), 227 g
Key Features and Details
EMM Nitrogen (no NH4Cl, Glutamate) is a minimal defined medium for the growth of S. pombe, containing dextrose, buffers, salts, vitamins and trace minerals. EMM minimal defined medium is thiamine-free.
SYNONYMS: Edinburgh's Minimal Medium No Nitrogen |S.pombe growth medium
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PRODUCT DESCRIPTION
EMM Nitrogen (No NH4Cl, Glutamate) is a minimal defined medium for the growth of S. pombe, containing dextrose, buffers, salts, vitamins and trace minerals. EMM minimal defined medium is thiamine-free nmt1 promoter studies in Schizosaccharomyces pombe.
Application Notes
EMM w/o Nitrogen is used for the growth of S.pombe.
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
Cell culture |Proteomics |Purification and Detection |Protein Analysis |Yeast Culture |Plasmid Production |Protein Expression |Gene Expression |Cloning |Gene Function |Gene Regulation |Automated Bacterial Growth Media |S. pombe Culture
SPECIFICATIONS
SKU: 114110712
Base Catalog Number: 4110712
Alternate Names: Edinburgh's Minimal Medium No Nitrogen |S.pombe growth medium
Format
: Powder
Formulation Details
: Contents Per Liter: 20 g d-glucose; 3 g potassium hydrogen phthalate (14.7 mM); 2.2 g Na2HPO4 (15.5 mM); 1.05 g MgCl2.6H2O (5.2 mM); 14.7 µg CaCl2.2H2O (99.8 µM); 1 g KCl (13.4 mM); 0.04 g Na2SO4 (0.28 mM); 1mg pantothenic acid (4.2 µM); 10 mg nicotinic acid (81.2 µM); 10 mg myo-inositol (55.5 µM); 10 µg biotin (0.82 µM); 0.5 mg boric acid (8.9 µM); 0.4 mg MnSO4 (2.4 µM); 0.4 mg ZnSO4.7H2O (1.4 µM); 0.2 mg FeCl2.6H2O (0.7 µM); 40 µg molybdic acid (0.25 µM); 0.1 mg KI (0.6 µM); 40 µg CuSO4.5H2O (0.016 µM); 1 mg citric acid (4.76 µM).
Grade
: Molecular Biology Certified
Media Type
: EMM
pH
: pH Range: 5.4 - 6.4 at 25 °C
PPE
: Personal Protective Equipment Respiratory protection: Breathing apparatus only if aerosol or dust is formed. Hand protection: PVC or other plastic material gloves Skin and body protection: Usual safety precautions while handling the product will provide adequate protection against this potential effect. Eye protection: Safety glasses with side-shields Hygiene measures: Handle in accordance with good industrial hygiene and safety practice
Preparation Method
: Reagents are tested and chosen for their ability to promote optimum growth of S. pombe when combined in EMM Broth formulations. Chosen reagents are combined in the proper proportions according to the formulation listed above and thoroughly blended for a uniform distribution. A sample of the blended formulation is used to prepare agar medium. The combinations are mixed to dissolve components and then autoclaved at 121 °C for 15 minutes.
Solubility
: Soluble in water (27 g/L).
Sterilization of Solutions
: Autoclaved at 121 °C for 15 minutes. Note: Autoclaving at higher temperatures or for longer periods will result in darkening of the sterile medium, (a darker color does not normally affect growth of cells, only the appearance of the sterile medium).
