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Hyaluronidase from bovine testes, =300 USP U/mg
Key Features and Details
Hyaluronidase
SYNONYMS: Hyaluronate Glycanohydrolase; Hyaluronate lyase; chondroitinase; :Hyaluronoglucosaminidase Hyaluronate 4-glycanohydrolase
CAS: #
37326-33-3
EC No: 253-464-3
MDL No: MFCD00131351
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PRODUCT DESCRIPTION
Hyaluronidase
Application Notes
Testicular hyaluronidase hydrolyzes the endo-N-acetylhexosaminic bonds of hyaluronic acid and chondroitin sulfuric acids A and C (but not B), primarily to tetrasaccharide residues. Monosaccharides are not liberated. Hyaluronidase is often used in conjunction with collagenase to dissociate the extracellular matrix between cells of animal tissue, in order to release viable cells for use in tissue culture. It may also be used to clarify synovial fluids in order to make cell counts possible.
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
Drug dispersion & delivery
SPECIFICATIONS
SKU: 02100740-CF
Base Catalog Number: 100740
Alternate Names: Hyaluronate Glycanohydrolase; Hyaluronate lyase; chondroitinase; :Hyaluronoglucosaminidase Hyaluronate 4-glycanohydrolase
Biochemical Physiological Actions
: Hyaluronidase degrades hyaluronan and has been found to be inappropriately regulated during cancer progression. These enzymes randomly cleave ß-N-acetylhexosamine-[1?4] glycosidic bonds in hyaluronic acid, chondroitin, and chondroitin sulfates.
CAS
: #
37326-33-3
EC No
: 253-464-3
Extinction Coefficient
: ~8 (lit.)
Format
: Lyophilized powder form
Grade
: USP
Hazard Statements
: H317
MDL No
: MFCD00131351
PPE
: Eyeshields, Gloves, Respirator filter
Source
: Bovine Testes
Specific Activity
: =300 USP u/mg
Specificity
: Testicular hyaluronidase hydrolyzes the endo-N-acetylhexosaminic bonds of hyaluronic acid and chondroitin sulfuric acids A and C (but not B); primarily to tetrasaccharide residues. Monosaccharides are not liberated
Unit Definition
: Amount of enzyme which liberates one micromole of N-acetylglucosamine per minute at 37°C and pH 4.0
