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Isis DNA polymerase (1 U/µl)
Key Features and Details
For high fidelity PCR – Extremely low error rates – Optimal blunt end cloning – The most thermostable polymerase available
SYNONYMS: High Fidelity Polymerase |Proofreading DNA Polymerase
EC No: 232-741-2
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PRODUCT DESCRIPTION
Isis DNA Polymerase is a proofreading DNA polymerase and amplifies DNA with 40 times more accuracy than standard Taq DNA polymerase.
Application Notes
Ideal for high-fidelity amplification |Use to amplify DNA with difficult to read secondary structures |Optimal for blunt-end PCR cloning |The most thermostable polymerase available |Useful to amplify GC rich sequences
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
Molecular Biology |PCR/Amplification |PCR |cDNA synthesis |RT-PCR |DNA labeling |High Fidelity PCR
SPECIFICATIONS
SKU: 11EPSIS100-CF
Base Catalog Number: EPSIS100
Alternate Names: High Fidelity Polymerase |Proofreading DNA Polymerase
Brand
: Isis
Components
: 100 U Isis DNA Pol at 1 U/µL |Isis buffer 10x C (1ml) with MgSO4 |1 mL MgSO4 at 30 mM
Concentration
: 1 U/µl
EC No
: 232-741-2
Format
: Kit
Grade
: Molecular Biology
PPE
: Personal-protective-equipment: If working with biological, toxic, hazardous or infectious samples; Hand protection: Latex, Nitrile or other plastic material gloves. Skin and body protection: Labcoat and sleves. Eye protection: Safety glasses with side-shields Hygiene measures: Handle in accordance with good industrial hygiene and safety practice.
Protein or Enzyme Type
: Molecular Biology Enzymes
References
: (1) Flaman, J.M., et al. (1994) Nucl. Acids Res. 15, 3259-3260 |(2) Flaman, J.M., et al. (1995) Proc. Natl. Acad. Sci. USA 92, 3963-3967 |(3) Gueguen, Y. et al., (2001) Eur. J. Biochem. 268, 1-10 |(4) Gueguen, Y. et al., (2002) FEMS Microbiology Letters. 217, 89-94
Unit Definition
: One unit is the amount of enzyme required to catalyse the incorporation of 10 nmol of nucleotides into acid insoluble material in 30 min. at 74 °C under assay conditions.
