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LB medium, pouches
Key Features and Details
Pre-measured, sealed pouches, for 0.5 L or 1 L batch sizes, simply tear open the pouch and add to water.
SYNONYMS: Luria Bertani Medium |Luria Bertani Broth |Luria Broth Base Miller Formulation
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PRODUCT DESCRIPTION
LB medium is the most widely used medium for the growth of bacteria. MP Biomedicals offers LB media in a variety of formats for convenience and ease of use. It is a rich Medium for the growth of E. coli and certified for plasmid and other bacterial cultures in liquid medium. MP Biomedicals offers LB media in pre-measured pouches for convenience and ease of use.
Application Notes
LB Medium is a nutritionally rich medium originally developed for growth and maintenance of E. coli strains. LB Medium supports a luxurious growth of E. coli cells as tryptone and yeast extract supply essential growth factors that E. coli cells would otherwise have to synthesize. The medium also contains essential electrolytes for transport and osmotic balance, due to the NaCl component .
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
Cell culture |Proteomics |Purification and Detection |Recombinant Protein Expression and Analysis |Bacterial Culture |Plasmid Production |Protein Expression |Gene Expression |Cloning |Genetic Manipulation
SPECIFICATIONS
SKU: 113002065-CF
Base Catalog Number: 3002065
Alternate Names: Luria Bertani Medium |Luria Bertani Broth |Luria Broth Base Miller Formulation
Format
: Pouch
Formulation Details
: Typically contain 10 g of tryptone and 5 g of yeast extract per liter; and vary in salt. Small quantities of biological buffers have been added for proper pH adjustment.
Grade
: Molecular Biology Certified
Media Type
: LB
pH
: Acceptable pH range is 6.5 - 7.5 at 25 °C.
PPE
: Personal Protective Equipment Respiratory protection: Breathing apparatus only if aerosol or dust is formed. Hand protection: Pvc or other plastic material gloves Skin and body protection: Usual safety precautions while handling the product will provide adequate protection against this potential effect. Eye protection: Safety glasses with side-shields Hygiene measures: Handle in accordance with good industrial hygiene and safety practice.
Preparation Method
: Media Preparation: Reagents are tested and chosen for their ability to promote optimum growth of E. coli when combined in LB Medium formulations. Chosen reagents are combined in the proper proportions according to the LB Medium formulation and thoroughly blended for a uniform distribution. A sample of the blended formulation is used to prepare liquid medium. The combinations are mixed to dissolve components and then autoclaved at 121 °C for 15 minutes.
Solubility
: Soluble in water (25g/L).
Sterility
: Sterile
Typical Working Concentration
: 25g/L
