No products in the cart.
Lysozyme, =20,000 U/mg solid
Key Features and Details
Lysozyme from chicken egg white Active over a broad pH range (6.0 to 9.0) and ionic strength (0.02 to 0.1 M) Enzyme breaks down the cell walls of bacteria
SYNONYMS: Muramidase; Mucopeptide N-acetylmuramoylhydrolase
CAS: #
9001-63-2
MOLECULAR WEIGHT: 2235.597 g/mol
EC No: 232-620-4
MDL No: MFCD00131557
You need to register an account with us
to be able to place an order, Click to Register.
PRODUCT DESCRIPTION
Lysozyme
Application Notes
Lysozyme has been used for lysing E. coli and Streptomycetes for extraction purposes such as extracting group specific antigen. It would appear that lysozyme may act as a germinative agent of bacterial spores.In humans, lysozyme may be the mediator in the anti-tumor function of macrophages which, it has been shown, secrete the enzyme. There is evidence that cartilage lysozyme has a role in cartilage calcification. Enzyme breaks down the cell walls of bacteria; used to prepare spheroplasts.
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
Cell Lysis
SPECIFICATIONS
SKU: 02100831-CF
Base Catalog Number: 100831
Alternate Names: Muramidase; Mucopeptide N-acetylmuramoylhydrolase
Biochemical Physiological Actions
: Lysozyme hydrolyzes ß(1?4) linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrin. Gram-positive cells are quite susceptible to this hydrolysis as their cell walls have a high proportion of peptidoglycan. Gram-negative bacteria are less susceptible due to the presence of an outer membrane and a lower proportion of peptidoglycan. However, these cells may be hydrolyzed in the presence of EDTA that chelates metal ions in the outer bacterial membrane. The enzyme is active over a broad pH range (6.0 to 9.0). At pH 6.2, maximal activity is observed over a wider range of ionic strengths (0.02 to 0.100 M) than at pH 9.2 (0.01 to 0.06 M).
CAS
: #
9001-63-2
EC No
: 232-620-4
Extinction Coefficient
: 26.4 (280 nm) (Lit.)
Format
: lyophilized, Salt-free, 3X Crystallized, Albumin-Free
Isoelectric Point
: pH 11.0 (lit)
MDL No
: MFCD00131557
Molecular Weight
: 2235.597 g/mol
PPE
: Eyeshields, Gloves, respirator filter
RTECS No
: OL5987000
Source
: Chicken eggwhite
Specific Activity
: =20,000 u/mg solid
Unit Definition
: One unit will cause a decrease in A450 of 0.001 per minute at pH 6.24 and 25 °C using Micrococcus lysodeikticus as substrate.
