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Lysozyme, =9000 Sugar U/mg solid, from chicken eggwhite
Key Features and Details
Lysozyme
SYNONYMS: Muramidase; Mucopeptide N-acetylmuramoylhydrolase
CAS: #
9001-63-2
MOLECULAR WEIGHT: 2235.597 g/mol
EC No: 235-747-3
MDL No: MFCD00131557
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PRODUCT DESCRIPTION
Lysozyme
Application Notes
Lysozyme has been used for lysing E. coli and Streptomycetes for extraction purposes such as extracting group specific antigen. It would appear that lysozyme may act as a germinative agent of bacterial spores. The enzyme is often used for lysing bacterial cells by hydrolyzing the peptidoglycan present in the cell walls. Used to prepare spheroplasts.
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
Germinative agent | Extracting group specific antigen
SPECIFICATIONS
SKU: 02100834-CF
Base Catalog Number: 100834
Alternate Names: Muramidase; Mucopeptide N-acetylmuramoylhydrolase
Biochemical Physiological Actions
: Lysozyme hydrolyzes ß(1?4) linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrin. Gram-positive cells are quite susceptible to this hydrolysis as their cell walls have a high proportion of peptidoglycan. Gram-negative bacteria are less susceptible due to the presence of an outer membrane and a lower proportion of peptidoglycan. However, these cells may be hydrolyzed in the presence of EDTA that chelates metal ions in the outer bacterial membrane.
CAS
: #
9001-63-2
EC No
: 235-747-3
Extinction Coefficient
: 26.4 (Lit.)
Format
: Crystalline powder
Hazard Statements
: H317
Isoelectric Point
: 11.0(Lit.)
MDL No
: MFCD00131557
Molecular Weight
: 2235.597 g/mol
PPE
: Eyeshields, Gloves, respirator filter
RTECS No
: OL5987000
Source
: Chicken eggwhite
Specific Activity
: =9000 Sugar U/mg solid
Unit Definition
: One unit will cause a decrease in OD450 of 0.001 per minute at pH 6.24 and 25 °C using Micrococcus lysodeikticus as substrate.
