M9 minimal medium salts

M9 Minimal Salts are a commonly used base for preparing a defined synthetic medium for the growth of most E. coli host cells. The powder is reconstituted in sterile purified water, (or water with melted purified agar added.) Additional nutrients, such as glucose or other carbon source, casamino acids, vitamins, or antibiotics are added when the solution cools and before plates are poured or media is inoculated with bacteria. Application Notes M9 Minimal medium salts are suitable for the preparation of M9 Minimal Medium for use in propagation of E. coli and plasmid amplification. The resulting M9 medium can be used for maintaining positive selection pressure on plasmids coding for genes to produce essential substances such as amino acids and vitamins and for maintenance of F’-containing bacteria strains for M13 propagation. Usage Statement Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. Key Applications Cell culture |Proteomics |Purification and Detection |Recombinant Protein Expression and Analysis |Bacterial Culture |Plasmid Production |Protein Expression |Gene Expression |Cloning |Genetic Manipulation |Growth and Maintenance of M13 phage & other filamentous ssDNA bacteriophages

Formulation Details : 33.9 g Na2HPO4; 15 g KH2PO4; 2.5 g NaCl; 5 g NH4Cl = 56.4 g/L of a 5x solution.