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Protein A from Staphylococcus aureus, 20 mg
Key Features and Details
Protein A
SYNONYMS: Protein A from Staphylococcus aureus
CAS: #
Not applicable
MDL No: MFCD00081934
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PRODUCT DESCRIPTION
Protein A
Application Notes
Protein A may be conjugated with various reporter molecules including fluorescent dyes (FITC), enzyme markers (peroxidase, ß-galactosidase, alkaline phosphatase), biotin, and colloidal gold without affecting the antibody binding site on the molecule. These conjugates are used to detect immunoglobulins in various immunochemical assays including western blotting, immunohistochemistry, and ELISA applications. In addition, protein A may be immobilized on a solid support such as agarose or acrylic beads for the purification of either polyclonal or monoclonal immunoglobulins. It is also routinely used for immunoprecipitation assays.
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
Detection of immunoglobulins in various immunochemical assays | Purification of either polyclonal or monoclonal immunoglobulins
SPECIFICATIONS
SKU: 0215005020
Base Catalog Number: 15005020
Alternate Names: Protein A from Staphylococcus aureus
Biochemical Physiological Actions
: Protein A is capable of binding to the Fc portion of immunoglobulins, especially IgGs, from a large number of species. One protein A molecule has been shown to bind at least 2 molecules of IgG simultaneously. The IgG binding domain of Protein A consists of three anti-parallel a-helicies, the third of which is disrupted when the protein is complexed with the Fc region of the immunoglobulins. Protein A will bind the Fc portion of human IgG subclasses, IgM, IgA and IgE; and mouse IgG1 (weakly), IgG2a, and IgG2b. Protein A also binds IgGs from other species, including monkey, rabbit, pig, guinea pig, dog, and cat. Protein A also participates in a number of different protective biological functions including anti-tumor, toxic, and carcinogenic activities. In addition to acting as an immunomodulator, it also has antifungal and antiparasitic properties.
Capacity
: Binding Capacity: 6-8 mg of human IgG per mg solid(Lit.)
CAS
: #
Not applicable
Extinction Coefficient
: E1%280 = 1.4 (Lit.)
Format
: Frozen Solid
Isoelectric Point
: 4.85 - 5.10(Lit.)
MDL No
: MFCD00081934
PPE
: Eyeshields, Gloves, respirator filter
Source
: Staphylococcus aureus
Specificity
: Protein A will bind the Fc portion of human IgG subclasses; IgM; IgA and IgE; and mouse IgG1 (weakly); IgG2a and IgG2b. Protein A also binds IgGs from other laboratory and domestic animals; including monkey; rabbit; pig; guinea pig; dog and cat.
