ß-Nicotinamide adenine dinucleotide reduced, disodium salt

ß-Nicotinamide Adenine Dinucleotide Reduced, Disodium Salt Application Notes ß-Nicotinamide adenine dinucleotide (NAD+) and ß-Nicotinamide adenine dinucleotide, reduced (NADH) comprise a coenzyme redox pair (NAD+:NADH) involved in a wide range of enzyme catalyzed oxidation reduction reactions. In addition to its redox function, NAD+/NADH is a donor of ADP-ribose units in ADP-ribosylaton (ADP-ribosyltransferases; poly(ADP-ribose) polymerases ) reactions and a precursor of cyclic ADP-ribose (ADP-ribosyl cyclases). Many metabolites and enzymes of biological interest are present in tissues at low concentrations. With the use of ß-NADH as a cofactor and several enzymes in a multistep system, known as enzyme cycling, much greater sensitivity for detection of these components is achieved. ß-NADH is fluorescent; whereas, ß-NAD+ is not. This difference in fluorescence provides a sensitive fluorescent measurement of the oxidized or reduced pyridine nucleotides at concentrations down to 10-7 M. Discussion of optimizing the fluorescence intensity and identification of interfering substances has been reported. Usage Statement Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. Key Applications Coenzyme