SC-ADE-HIS-LEU-LYS-TRP-URA, 30 g

4+ dropout formulation (without Adenine, L-Histidine, L-Leucine, L-Lysine, L-Tryptophan and Uracil) of Synthetic Complete Supplement Mixture (SC) of amino acids for S. cerevisiae growth media. Also known as Hopkins Mixture and is available in complete and dropout formulations. Application Notes The selection of plasmids in yeast is based on the use of auxotrophic mutant strains that cannot grow without a specific media component (an amino acid, purine or pyrimidine). Transformation with a plasmid containing the mutated gene enables the transformant to grow on a medium lacking the required component. This SC-Dropout medium create a richer medium for better yield and growth rate, and increase the probability of successful transformations when screening libraries or performing gene knock-outs. Usage Statement Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. Key Applications Cell culture |Proteomics |Purification and Detection |Protein Analysis |Yeast Culture |Plasmid Production |Protein Expression |Gene Expression |Cloning |Gene Function |Gene Regulation |Automated Bacterial Growth Media |S. cerevisiae Culture

Formulation Details : Contents per Liter: Adenine 0 mg; L-Alanine 86.5 mg; L-Arginine HCl 85.6 mg; L-Asparagine 85.6 mg; L-Aspartic Acid 85.6 mg; L-Cysteine HCl 85.6 mg; Glutamine 85.6 mg; L-Glutamic Acid 85.6 mg; Glycine 85.6 mg; L-Histidine HCl 0 mg; Myo-Inositol 85.6 mg; L-Isoleucine 85.6 mg; L-Leucine 0 mg; L-Lysine HCl 0 mg; L-Methionine 85.6 mg; Para-Aminobenzoic Acid 8.6 mg; L-Phenylalanine 85.6 mg; L-Proline 85.6 mg; L-Serine 85.6 mg; L-Threonine 85.6 mg; L-Tryptophan 0 mg; L-Tyrosine 85.6 mg; Uracil 0 mg; L-Valine 85.6 mg; Total 2000.6 mg