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Tricine
Key Features and Details
Tricine
SYNONYMS: N-Tris-[hydroxymethyl]methylglycine; N-[2-Hydroxy-1,1-bis(hydroxymethyl) ethyl] glycine
CAS: #
1389475
MOLECULAR FORMULA: C6H13NO5
BEILSTEIN REGISTRY No.: 1937804
EC No: 227-193-6
MDL No: MFCD00004277
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PRODUCT DESCRIPTION
Tricine
Application Notes
Tricine can be used in cryopreservation medium for the preservation of tissues and organs. Cryopreservation depends on the physical and chemical characteristics of the preservation medium used. Tricine has been found to be an efficient scavenger of hydroxyl radicals in a study of radiation-induced membrane damage. It is typically the buffer of choice in SDS-PAGE systems when separating proteins in the range of 1 to 100 kDa.
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
Buffer | Cryopreservation medium | Scavenger of hydroxyl radicals
SPECIFICATIONS
SKU: 02103112-CF
Base Catalog Number: 103112
Alternate Names: N-Tris-[hydroxymethyl]methylglycine; N-[2-Hydroxy-1,1-bis(hydroxymethyl) ethyl] glycine
Beilstein Registration No
: 1937804
CAS
: #
1389475
EC No
: 227-193-6
Format
: Powder
MDL No
: MFCD00004277
Molecular Formula
: C6H13NO5
pKa
: 8.1 at 25 °C(Lit.)
PPE
: Eyeshields, Gloves, respirator filter
Sterilization of Solutions
: To sterilize solutions, MP recommends filter sterilization using a 0.2 micron filter.
Typical Working Concentration
: A buffer may be prepared by titrating with sodium hydroxide to the desired pH, using about a half-equivalent of NaOH.For ATP assays using firefly luciferase, tricine buffer at 25 mM was found to be the best of ten common buffers tested.
