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Urea, ultra pure, =99%, 10 kg
Key Features and Details
Urea is the principal end product of nitrogen metabolism in most mammals, formed by the enzymatic reactions of the Kreb’s cycle.
SYNONYMS: Carbamide; Isourea; Carbonyldiamide; Carbonyldiamine; Ureophil; Carbamimidic acid; Pseudourea; Ureaphil; Urevert
CAS: #
57-13-6
MOLECULAR FORMULA: CH4N2O
MOLECULAR WEIGHT: 60.06
BEILSTEIN REGISTRY No.: 635724
EC No: 200-315-5
MDL No: MFCD00008022
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PRODUCT DESCRIPTION
Urea is the principal end product of nitrogen metabolism in most mammals, formed by the enzymatic reactions of the Kreb's cycle.
Application Notes
Urea is a mild agent usually used in the solubilization and denaturation of proteins. It is also useful for renaturing proteins from samples already denatured with 6 M guanidine hydrochloride such as inclusion bodies; and in the extraction of the mitochondrial complex. It is commonly used to solubilize and denature proteins for denaturing isoelectric focusing and two-dimensional electrophoresis and in acetic acid-urea PAGE gels. Urea is used in cell or tissue culture media to increase the osmolality. Urea has also been used as fertilizer because of the easy availability of nitrogen; in animal feeds; it is reacted with aldehydes to make resins and plastics; condensed with malonic ester to form barbituric acid; used in the paper industry to soften cellulose; used as a diuretic; enhances the action of sulfonamides; an antiseptic.
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
Denaturation; Protein Electrophoresis; SDS-PAGE
SPECIFICATIONS
SKU: 04821858
Base Catalog Number: 821858
Alternate Names: Carbamide; Isourea; Carbonyldiamide; Carbonyldiamine; Ureophil; Carbamimidic acid; Pseudourea; Ureaphil; Urevert
Beilstein Registration No
: 635724
Biochemical Physiological Actions
: Urea in solution is in equilibrium with ammonium cyanate. The form that reacts with protein amino groups is isocyanic acid. Urea in the presence of heat and protein leads to carbamylation of the proteins. Carbamylation by isocyanic acid interferes with protein characterization because isocyanic acid reacts with the amino terminus of proteins, preventing N-terminal sequencing. Isocyanic acid also reacts with side chains of lysine and arginine residues resulting in a protein that is unsuitable for many enzymatic digests. In addition, carbamylation often leads to confusing results from peptides having unexpected retention times and masses. When performing enzymatic protein digests it is important to remove urea first.Even though some enzymes will tolerate small amounts of urea, the elevated temperature used for most reactions will lead to carbamylation during the course of the digest. The urea can be removed prior to digestion by fast reversed phase chromatography, spin columns, or dialysis.
Boiling Point
: Decomposes at 135 °C
CAS
: #
57-13-6
Density
: 1.335 g/cm3 at 20 °C
EC No
: 200-315-5
Grade
: Ultra Pure
MDL No
: MFCD00008022
Melting Point
: 130 - 140°C
Molecular Formula
: CH4N2O
Molecular Weight
: 60.06
Partition Coefficient
: log Pow: -2,59 - -1,59
pH
: 8.5 ± 1.0 (7M aq soln)
Preparation Method
: Dissolve urea in deionized water to the desired concentration.For every 10 ml of solution, add 1 g of Amberlite® IRA-910.Stir for one hour at room temperature
Purity
: =99%
RTECS No
: YR6250000
Solubility
: One gram dissolves in 1 mL water, 10 mL 95% ethanol, 1 mL boiling 95% ethanol, 20 mL absolute ethanol, 6 mL methanol, 2 mL glycerol; Soluble in concentrated hydrochloric acid; almost insoluble in chloroform, ether.
Typical Working Concentration
: Urea is typically used at a concentration of 8 M for protein denaturation or solubilization. A final concentration of 5 M urea is commonly used in molecular biology for sequencing gels.
UV Visible Absorbance
: OD280 (7M aq soln) =0.05
