The BcaBEST RNA PCR Kit is designed to perform both reverse transcription and DNA amplification in a single tube. This two-step RT-PCR kit takes advantage of the BcaBEST DNA Polymerase—which contains both DNA polymerase and reverse transcriptase properties—to enable first-strand cDNA synthesis. Compared with standard reverse transcriptases, BcaBEST polymerase operates at an optimal temperature of 65°C, permitting RT-PCR of long or difficult RNA templates. RT-PCR troubleshooting for highly structured RNA templates is especially recommended using the BcaBEST RNA PCR Kit. Bca-optimized Taq polymerase is used for second-strand synthesis and subsequent PCR. The Bca-optimized Taq polymerase utilizes LA PCR technology for improved PCR amplicon length and accuracy. Random 9-mers, oligo(dT) primers, or specific downstream primers (that function as antisense PCR primers) can be used for cDNA synthesis.