The PrimeScript 1st strand cDNA Synthesis Kit contains all of the reagents necessary for synthesis of full-length first-strand cDNA from total or poly A+ RNA, including both oligo(dT) and random 6-mer primers. This kit is powered by PrimeScript Reverse Transcriptase (PrimeScript RT), which has exceptionally strong strand-displacement activity and efficiently synthesizes cDNA. PrimeScript RT is robust, versatile, and well-suited for applications requiring full-length cDNA such as cDNA library construction. This PrimeScript-based cDNA synthesis kit also works well with other cDNA-dependent techniques, including RT-PCR, cDNA probe preparation, and quantitative PCR (qPCR). The PrimeScript 1st strand cDNA Synthesis Kit is ideal for reverse transcription of many different RNA templates, including GC-rich templates and RNAs with high levels of secondary structure.