This one-step RT-PCR kit allows efficient, specific cDNA synthesis and amplification in a single tube for endpoint analysis (e.g., gel electrophoresis). The kit features a streamlined workflow that does not require addition of reagents between the cDNA synthesis and amplification steps. The PrimeScript One Step RT-PCR Kit includes PrimeScript RT, an RNAse H-minus MMLV reverse transcriptase that allows robust reverse transcription of almost any RNA template, including GC-rich or highly structured RNA. To enable cDNA amplification, this one-step RT-PCR kit also includes Takara Ex Taq hot-start (HS) DNA polymerase, a thermostable polymerase that allows robust, specific amplification and is formulated for hot-start PCR. Thanks to the PrimeScript One Step Enzyme Mix, sample handling time and the risk of contamination are minimized. The PrimeScript One Step RT-PCR Kit (Dye Plus) also includes electrophoresis loading dyes (blue and yellow) in the premix to further reduce sample handling steps and allow visual confirmation of gel loading.