PrimeScript Reverse Transcriptase (PrimeScript RT), included in the PrimeScript High Fidelity RT-PCR Kit, efficiently synthesizes cDNA thanks to its exceptionally strong strand-displacement activity. PrimeScript RT is a modified, recombinant RNase H Minus MMLV (Moloney Murine Leukemia Virus) reverse transcriptase that is robust, versatile, and well-suited for applications requiring full-length cDNA, such as RT-PCR gene expression studies. PrimeScript RT can be used for reverse transcription of virtually any RNA template, including templates that are GC-rich and/or have high levels of secondary structure. Because of the excellent extension capability of this enzyme, cDNA synthesis can be performed at 42°C, decreasing the risk of RNA degradation that often occurs during conventional reactions performed at higher temperatures.