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Two-step RT-PCR
The RNA PCR Kit (AMV) Version 3.0 combines AMV Reverse Transcriptase with Takara Ex Taq HS—a high-fidelity, hot-start DNA polymerase—enabling users to synthesize and amplify cDNA in a single tube, using a two-step protocol. The RNA PCR kit uses AMV RTase XL, which possesses higher thermostability and a broader range of reaction temperatures (42–60°C) than MMLV RT. This allows transcription to occur at higher temperatures and helps eliminate potential RNA secondary structures that can impede downstream cDNA amplification. The supplied Oligo (dT) Adaptor primer is designed for highly efficient cDNA synthesis from the poly A+ RNA 3'-terminus, allowing later amplification of unknown 3'-termini using 3'-RACE. Components for 3'-RACE are supplied in this two-step RT-PCR kit.
The RNA PCR Kit (AMV) Version 3.0 combines AMV Reverse Transcriptase with Takara Ex Taq HS—a high-fidelity, hot-start DNA polymerase—enabling users to synthesize and amplify cDNA in a single tube, using a two-step protocol. The RNA PCR kit uses AMV RTase XL, which possesses higher thermostability and a broader range of reaction temperatures (42–60°C) than MMLV RT. This allows transcription to occur at higher temperatures and helps eliminate potential RNA secondary structures that can impede downstream cDNA amplification. The supplied Oligo (dT) Adaptor primer is designed for highly efficient cDNA synthesis from the poly A+ RNA 3′-terminus, allowing later amplification of unknown 3′-termini using 3′-RACE. Components for 3′-RACE are supplied in this two-step RT-PCR kit.
Product Ordering
Description & Specifications
Product Ordering
| SKU | Products | Size | Stock | Price | Quantity | Add to Cart |
|---|---|---|---|---|---|---|
| RR019A | RNA PCR Kit (AMV) Version 3.0 | 100 Rxns | In Stock | |||
| RR019B | RNA PCR Kit (AMV) Version 3.0 | 200 Rxns | In Stock |
Description & Specifications
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