While we continue to maintain our In-Fusion HD Cloning Plus kits, we strongly recommend that you switch to the more recently developed In-Fusion Snap Assembly master mixes. These newer versions provide the same robustness and reliability you have come to expect from In-Fusion technology, but are optimized for enhanced cloning performance. In-Fusion HD Cloning Plus products enable directional, seamless cloning of any PCR fragment into any linearized vector in a single 15-minute reaction.