E. coli DNA Ligase is a ligation enzyme that can be used to join DNA fragments by catalyzing the formation of phosphodiester bonds between double-stranded DNA fragments containing juxtaposed 5'-phosphate termini and 3'-hydroxyl termini in the presence of the NAD cofactor. Both E.coli DNA Ligase and T4 DNA Ligase are used in gene cloning experiments; however, E. coli DNA Ligase can only catalyze ligation of cohesive end-containing DNA fragments via standard reaction conditions.
E. coli DNA Ligase is a ligation enzyme that can be used to join DNA fragments by catalyzing the formation of phosphodiester bonds between double-stranded DNA fragments containing juxtaposed 5′-phosphate termini and 3′-hydroxyl termini in the presence of the NAD cofactor. Both E.coli DNA Ligase and T4 DNA Ligase are used in gene cloning experiments; however, E. coli DNA Ligase can only catalyze ligation of cohesive end-containing DNA fragments via standard reaction conditions.