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DNA Blunting Kit
The DNA Blunting Kit allows the conversion of 3' and 5' overhangs to blunt or flush ends. This conversion is accomplished simultaneously by the 3'→5' exonuclease and 5'→3' polymerase activities of T4 DNA Polymerase. The resulting blunt-ended DNA can then be ligated into a vector using the same optimized buffer system employed in our: DNA Ligation Kit, Mighty Mix; TaKaRa DNA Ligation Kit LONG; and Quick DNA ligation kit products. The reaction can then be used directly in bacterial transformation or in vitro packaging procedures without the need for further DNA purification.
The DNA Blunting Kit allows the conversion of 3′ and 5′ overhangs to blunt or flush ends. This conversion is accomplished simultaneously by the 3’→5′ exonuclease and 5’→3′ polymerase activities of T4 DNA Polymerase. The resulting blunt-ended DNA can then be ligated into a vector using the same optimized buffer system employed in our: DNA Ligation Kit, Mighty Mix; TaKaRa DNA Ligation Kit LONG; and Quick DNA ligation kit products. The reaction can then be used directly in bacterial transformation or in vitro packaging procedures without the need for further DNA purification.
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Description & Specifications
Product Ordering
| SKU | Products | Size | Stock | Price | Quantity | Add to Cart |
|---|---|---|---|---|---|---|
| 6025 | DNA Blunting Kit | 20 Rxns | In Stock |
Description & Specifications
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