The LA PCR in vitro Cloning Kit is designed specifically for long-range PCR and cloning of plasmid, cDNA, or genomic DNA regions that contain a known sequence flanking one end. This cloning kit for long fragments uses the Takara LA Taq enzyme for longer amplification and improved fidelity. Amplification is accomplished via cassette-mediated PCR whereby long DNA fragments, such as those found in genomic DNA, are used without the need for time-consuming library construction and screening. Users will need to design and prepare primers corresponding to the known region of the DNA to be amplified.