Mighty Cloning Reagent Set (Blunt End) is designed to allow PCR products to be quickly cloned into blunt-end vectors with a simple method. The blunting and 5'-phosphorylation reactions are done simultaneously and in parallel, so both blunt- and -dA ended products can be prepared in a single reaction. This kit eliminates the need for pretreatment (such as enzyme inactivation, removal of unused dNTPs and primers, etc.) of PCR products.
Mighty Cloning Reagent Set (Blunt End) is designed to allow PCR products to be quickly cloned into blunt-end vectors with a simple method. The blunting and 5′-phosphorylation reactions are done simultaneously and in parallel, so both blunt- and -dA ended products can be prepared in a single reaction. This kit eliminates the need for pretreatment (such as enzyme inactivation, removal of unused dNTPs and primers, etc.) of PCR products.