The M13mp18 RF phage vector is suitable for M13 sequencing by the dideoxy sequencing method. Since the phage vector contains a multiple cloning site in the lacZ region, recombinant vectors can be easily verified via blue/white colony screening using agar plates containing IPTG and X-Gal. This phage vector can also directly be used in M13 sequencing reactions because it is processed as a single-stranded DNA from phage particles.