E. coli Exonuclease I is a 3' to 5' exonuclease for single-stranded DNA degradation, resulting in the production of 5'-phosphate mononucleotides from the 3'-hydroxyl termini of single-stranded DNA. This 3' to 5' exonuclease is highly specific for single-stranded DNA and does not react with double-stranded DNA or RNA. Exonuclease I is inactivated by heat treatment at 80°C for 15 minutes.
E. coli Exonuclease I is a 3′ to 5′ exonuclease for single-stranded DNA degradation, resulting in the production of 5′-phosphate mononucleotides from the 3′-hydroxyl termini of single-stranded DNA. This 3′ to 5′ exonuclease is highly specific for single-stranded DNA and does not react with double-stranded DNA or RNA. Exonuclease I is inactivated by heat treatment at 80°C for 15 minutes.