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Exonuclease III
Exonuclease III is a 3' to 5' exonuclease that removes mononucleotides from the 3'-hydroxyl termini of double-stranded DNA or DNA-RNA hybrids. Because a set number of mononucleotides are generated during each degradation, double-stranded DNA degradation is progressive. Exonuclease III will degrade double-stranded DNA containing a blunt end, a 5'-overhang, or a nick, but it will not degrade DNA with a 3'-overhang containing four or more bases. This 3' to 5' exonuclease can be inactivated by heating it at 65°C for 5 min. Exonuclease III is supplied in a buffer of 25 mM Tris-HCl (pH 8.0), 50 mM KCl, 0.5 mM DTT, and 50% glycerol.
Exonuclease III is a 3′ to 5′ exonuclease that removes mononucleotides from the 3′-hydroxyl termini of double-stranded DNA or DNA-RNA hybrids. Because a set number of mononucleotides are generated during each degradation, double-stranded DNA degradation is progressive. Exonuclease III will degrade double-stranded DNA containing a blunt end, a 5′-overhang, or a nick, but it will not degrade DNA with a 3′-overhang containing four or more bases. This 3′ to 5′ exonuclease can be inactivated by heating it at 65°C for 5 min. Exonuclease III is supplied in a buffer of 25 mM Tris-HCl (pH 8.0), 50 mM KCl, 0.5 mM DTT, and 50% glycerol.
Product Ordering
Description & Specifications
Product Ordering
| SKU | Products | Size | Stock | Price | Quantity | Add to Cart |
|---|---|---|---|---|---|---|
| 2170A | Exonuclease III | 5,000 Units | In Stock | |||
| 2170B | Exonuclease III | 25,000 Units | In Stock |
Description & Specifications
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