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T4 DNA Polymerase
T4 DNA Polymerase catalyzes the 5'→3' incorporation of nucleotides into double-stranded DNA using single-stranded and primed DNA as a template. It possesses strong 3'→5' exonuclease (proofreading) activity but does not exhibit 5'→3' exonuclease activity. Typical T4 DNA polymerase protocols include 5' DNA overhang blunting as well as the generation of blunt double-stranded DNA from double-stranded DNA containing 3' overhangs. These DNA products are often used in blunt cloning. T4 DNA Polymerase is supplied in a buffer of 200 mM potassium phosphate (pH 6.5), 10 mM DTT and 50% glycerol.
T4 DNA Polymerase catalyzes the 5’→3′ incorporation of nucleotides into double-stranded DNA using single-stranded and primed DNA as a template. It possesses strong 3’→5′ exonuclease (proofreading) activity but does not exhibit 5’→3′ exonuclease activity. Typical T4 DNA polymerase protocols include 5′ DNA overhang blunting as well as the generation of blunt double-stranded DNA from double-stranded DNA containing 3′ overhangs. These DNA products are often used in blunt cloning. T4 DNA Polymerase is supplied in a buffer of 200 mM potassium phosphate (pH 6.5), 10 mM DTT and 50% glycerol.
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Description & Specifications
Product Ordering
Description & Specifications
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