SfiI restriction site:
GGCCN NNN | NGGCC
CCGGN | NNN NCCGG
Supplied buffer: M
Effect of DNA methylation: enzyme activity is often affected by dcm methylase
Reaction temperature: 50°C
SfiI restriction site:
GGCCN NNN | NGGCC
CCGGN | NNN NCCGG
Supplied buffer: M
Effect of DNA methylation: enzyme activity is often affected by dcm methylase
Reaction temperature: 50°C