The NucleoSnap cfDNA kit is designed for isolating circulating cell-free DNA (cfDNA) from plasma. NucleoSnap columns are equipped with a predetermined breaking point and can be divided into a funnel component and a mini spin column by simply snapping off the funnel component. These columns allow for an ultra-fast procedure, with final elution from a mini spin column using a microcentrifuge after vacuum processing. NucleoSnap cfDNA columns are optimized for DNA isolation, with no need for carrier RNA.