Analysis of environmental DNA (eDNA) can provide a deeper understanding of freshwater and saltwater ecosystems, but common methods for eDNA isolation can be lengthy and laborious, often involving overnight incubation steps. The NucleoSpin eDNA Water kit is designed to streamline eDNA isolation workflows by providing a versatile solution that can be applied to filtered or unfiltered samples, featuring a protocol that can be completed in a little over one hour.